TB500 (Thymosin B4 Acetate) About This Product
TB500 is a synthetic research peptide modeled after thymosin beta-4, a naturally occurring protein found in many tissues. It has a reported molecular weight of approximately 4963 g/mol and contains a long peptide sequence often associated with cell structure, movement, and stress response research.
The TB500 peptide is supplied as a lyophilized (freeze-dried) powder in a convenient 10 mg vial, allowing researchers to prepare precise stock solutions for experimental use. The compound is produced at ≥99% HPLC-verified purity.
TB500 has been studied in laboratory models examining cell migration, cytoskeletal behavior, tissue organization, and responses to controlled injury or inflammation. All current data reflect preclinical research, and TB500 is not associated with clinical use.
TB500 (Thymosin B4 Acetate) Key Features and Benefits
- ≥99% HPLC-verified high-purity lyophilized peptide
- Synthetic structure supports consistency across experiments
- Has been studied for roles in cell movement, actin regulation, and tissue organization
- Supplied in a sealed TB500 10 mg vial for quality and sterility
- Suitable for in vitro, ex-vivo, and controlled animal model studies
- Stable under low-temperature storage and standard peptide-handling protocols
- For laboratory research use only
TB500 (Thymosin B4 Acetate) Mechanism & Research Applications
Research on the thymosin beta-4 peptide often focuses on its interactions with the cytoskeleton, especially with actin, one of the most abundant structural proteins in cells.
TB500 acts as an actin-binding and actin-sequestering peptide, meaning it binds to G-actin monomers and influences how they assemble into F-actin filaments. This regulation is important for how cells move, change shape, and repair structural damage.
As experts use TB500 for research, they commonly investigate:
- Cell migration and wound-model behavior
- Cytoskeletal reorganization
- Cellular responses to mechanical strain, inflammatory stimulation, or tissue injury
- Processes related to angiogenesis, stress signaling, and intracellular transport
Because its activity involves key structural and regulatory pathways, TB500 is used as a tool to help understand how cells adapt to internal and external stressors in controlled laboratory environments.
TB500 (Thymosin B4 Acetate) Dosing & Observed Effects in Research
Dosing in TB500 studies varies widely across models, organisms, and study designs.
- Cell-based assays: Typically 50 nM to 1 μM
- Animal models: Often 5–10 mg/kg loading doses. Dosing may occur weekly or according to study-specific intervals
Observed Experimental Endpoints
Reported findings include changes in:
- Cell migration rate
- Actin organization and cytoskeletal markers
- Inflammatory response readouts
- Tissue remodeling indicators
These observations reflect controlled research settings only and do not imply therapeutic application.
TB500 (Thymosin B4 Acetate) Storage, Safety & References
For proper storage, follow these guidelines:
- Store lyophilized peptide at –20°C or below
- Protect from light, moisture, and temperature fluctuations
- Shelf life typically 12–24 months when stored correctly
- Shipments are packaged with desiccants and cold-chain protection
Reconstitution Guidance
- Reconstitute with sterile water or 0.1M PBS (pH 7.4)
- Typical stock solution: 1 mg/mL
- Stable 24–48 hours at 4°C after reconstitution
- For longer storage: aliquot and freeze at –20°C (avoid repeated freeze–thaw cycles)
Safety Information
- Use standard PPE (gloves, coat, eye protection)
- Handle within sterile laboratory conditions
- Dispose of materials according to institutional biosafety guidelines
- MSDS available upon request
- Endotoxin testing is performed (<5 EU/mg)
- Not approved for human or veterinary use
References
https://pubmed.ncbi.nlm.nih.gov/22074294
https://pubmed.ncbi.nlm.nih.gov/14500546
https://pubmed.ncbi.nlm.nih.gov/11950239
https://pubmed.ncbi.nlm.nih.gov/25015963
Compliance Notice
This product is intended for laboratory research use only and is not approved for human or veterinary use.