Eyelashes play a crucial role in eye protection and aesthetics, with growth rates averaging 0.12–0.14 mm per day during the anagen stage. A study found that a bimatoprost ophthalmic solution of 0.03% effectively increased eyelash prominence when applied daily for four months, with significant regrowth in participants.

Latisse, which contains bimatoprost, is a treatment for enhancing eyelash growth. Understanding its dosage and application is essential for achieving desired results while minimizing potential side effects.

This article will delve into the specifics of Latisse dosage, application methods, benefits, and potential side effects, providing essential information for practitioners.

Key Takeaways

  • Latisse is a prescription treatment with a strength of 0.3 mg per mL used to make eyelashes grow longer and thicker by treating hypotrichosis.
  • Doctors recommend applying one drop of Latisse to the base of the upper eyelid once daily for 16 weeks for the best results.
  • Using a sterile applicator each time helps avoid infections and ensures effective treatment. Do not double up if you miss a day; apply it the next day.
  • Practitioners should adjust dosages based on patient responses and closely watch for side effects or adverse reactions.
  • Educating patients on proper application techniques and ensuring they follow instructions exactly as directed leads to optimal outcomes in safely achieving fuller lashes.

About: Medical Spa RX provides medical practices with premium products at the best prices. If you’re looking to buy Latisse for your practice, the sales representatives at Medical Spa RX can give you guidance.

Eye lash extensions.

Latisse, with a strength of 0.3 milligrams per mL, is the first FDA-approved prescription treatment to grow eyelashes for people who don’t have enough lashes. It works by treating hypotrichosis of the eyelashes, making them grow longer and thicker.

Doctors order and prescribe Latisse in their offices after explaining how to use it correctly. Patients should apply it daily for 16 weeks to see full results. Skipping a dose means simply waiting until the next day. Using it more often won’t help lashes grow faster or fuller but might increase the risk of side effects.

Eye lashes.

Apply one drop of Latisse to the base of the upper eyelid daily to treat hypotrichosis and promote eyelash growth. Use a new sterile applicator with each dose to prevent eye infection or injury. Continue this treatment for 16 weeks to achieve full results, with lashes becoming longer, thicker, and darker.

It’s important not to exceed the once-daily application, as using Latisse more frequently will not enhance results. If you miss a dose, apply it the next day at your regular time. Maintaining a clean application process ensures the best outcomes without complications.

Proper Application Techniques

Washing hands.

Proper application of Latisse is essential to good results. Keeping the process clean helps avoid eye infections and ensures the medication works well.

Here are some tips:

  • Always wash your hands with soap and water before touching the Latisse bottle or applicator.
  • Use a new, sterile applicator for each dose. This practice stops germs from spreading.
  • Do not let the tip of the bottle or applicator touch surfaces, including your hands or eyes.
  • Open the bottle carefully to prevent any contamination.
  • Apply Latisse only to the base of the upper eyelids. Avoid using it on lower lids to prevent it from spreading to unintended areas.
  • If you accidentally drop an applicator, throw it away. Use a fresh one instead.
  • Keep the bottle tightly closed when not in use to keep contaminants out.
  • Dispose of each applicator after a single use. Do not try to reuse them.
  • Instruct patients not to share their Latisse with others. Sharing can spread bacteria and infections.
  • Store Latisse at room temperature, away from moisture and heat, which can breed bacteria.

Latisse is a proven solution for enhancing eyelash growth. To treat hypotrichosis of the eyelashes, it is recommended that you apply it daily for 16 weeks. This treatment involves a precise dosage and application technique to ensure its effectiveness.

Dosage Adjustments and Monitoring

Patients may react differently to Latisse, making the initial prescription treatment crucial. Healthcare professionals must monitor patient responses to the daily 0.3 mg/mL strength solution.

If side effects occur or expected eyelash growth is not observed within 16 weeks, doctors might adjust the treatment frequency. Missed doses should be skipped rather than doubled up, as using Latisse more than once daily is not recommended.

Monitoring side effects and adverse reactions is critical when using Latisse, the first FDA-approved prescription for eyelash growth in patients with insufficient lashes. Practitioners should be familiar with the drug’s pharmacology and detailed dosing instructions to ensure patient safety and treatment effectiveness.

Clinical Guidelines and Best Practices

Educating patients about Latisse and its proper use is vital. Inform them that Latisse is a 0.3 mg/mL prescription treatment designed to grow longer, fuller, darker eyelashes. 

Specialists should emphasize the importance of applying the solution once daily for 16 weeks on the upper eyelid’s base using a sterile applicator. Explain that skipping doses or doubling up can negatively affect results. They can also discuss using Latisse in conjunction with other treatments, such as combining Latisse and lash extensions to achieve optimal results from the treatment.

Doctors should ensure patients follow the correct dosage, applying one drop daily. This careful approach helps effectively treat hypotrichosis. It is crucial to avoid using more than the recommended amount or trying to make up for missed doses, as this can cause issues and will not enhance results.


Knowing how to use Latisce right is vital for doctors. This guide shows the correct dose and ways to apply it, focusing on keeping things clean and watching out for any bad reactions.

It’s all about helping patients get fuller eyelashes safely. So, keep this info handy – it’s a big help in giving care that makes a difference.


1. How much Latisse should be applied for it to work?

Just a single drop on the applicator brush for each eye once a day.

2. Can I use Latisse more than once a day to get faster results?

No, using it more than once daily won’t speed up the results and isn’t recommended.

3. What happens if I miss a dose of Latisse?

If you miss a dose, apply it the next evening – no need to double up.

4. Is a specific time of day best for applying Latisse?

Yes, applying it at night works best after you’ve cleaned your face and removed any makeup.

5. Can I use mascara while also using Latisse?

You can wear mascara or other eye makeup while using Latisse; just put it on in the morning after the solution has dried overnight.

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Nguyen, B., Hu, J. K., & Tosti, A. (2022). Eyebrow and eyelash alopecia: A clinical review. American Journal of Clinical Dermatology, 23(4), 467-482. https://doi.org/10.1007/s40257-022-00729-5

Healthline. (n.d.). Latisse dosage. Retrieved from https://www.healthline.com/health/drugs/latisse-dosage

Drugs.com. (n.d.). Latisse [Prescription label information]. Retrieved from https://www.drugs.com/pro/latisse.html